ABSTRACT
Objective: To study the chemical constituents from the aerial parts of Glycyrrhiza uralensis. Methods: The compounds were isolated and purified by silica gel, Sephadex LH-20 column chromatography, and HPLC. The structures of the compounds were identified on the basis of chemical and spectral methods. Results: Twelve compounds were isolated from the ethanol extract of the the the aerial parts of G. uralensis and identified as (2S)-3'-(2-hydroxy-3-methylbut-3-enyl)-4',5,7-trihydroxy-dihydroflavanone (1), pinocembrin (2), sigmoidin B (3), licoflavanone (4), 6-prenylnaringenin (5), pinobanksin (6), galangin (7), genistein (8), pratensein (9), kaempferol-3-O-β-D-rutinoside (10), rutin (11), and α,α'-dihydro-3,5,3'-trihydroxy-4'-methoxy-5'-isopentenyl-stilbene (12). Conclusion: Compound 1 is a new compound named hydroxylicoflavanone, and compounds 3, 6, 7, and 9 are isolated from this plant for the first time.
ABSTRACT
Objective: To study the chemical constituents from the flower buds of Rosa rugosa. Methods: The chemical constituents from the flower buds of R. rugosa were isolated by silica gel, MCI-gel resin, Sephadex LH-20 column chromatography and high performance liquid chromatography (HPLC) methods. Their structures were elucidated by spectroscopic methods and physicochemical properties. Results: Three isoflavones, 6,8-dihydroxy-4',7-dimethoxyisoflavone (1), prunetin (2), and pratensein (3) were isolated from the flower buds of R. rugosa. Conclusion: Compound 1 is a new compound named rosa isoflavone. Compounds 2 and 3 are isolated from R. rugosa for the first time. Compound 1 displays the stronger cytotoxicity against A549 and PC3 cells with IC50 values of 2.6 and 3.2 μmol/L, respectively.
ABSTRACT
Objective: To study the chemical compounds from the leaves of Cajanus cajan. Methods: Normal phase silica gel, medium-pressure ODS, MCI, and Sephadex LH-20 gel column chromatographies were used to isolate and purify the constituents. And their structures were identified by their physical properties, nuclear magnetic resonance spectrum, mass spectrometry, etc. Results: Twenty-three compounds were separated from the alcohol extract in the leaves of C. cajan. They were cajanine (1), longistyline A (2), longistyline C (3), cajanolactone A (4), pinostrobin (5), orientin (6), isovitexin (7), vitexin (8), cajanol (9), cajanin (10), prunetin (11), pratensein (12), (2R, 3R)-2, 3-dihydro-5-7, 4'-dimethoxyflavone (13), ethyl 10', 16'-dihydroxy hexadecanoate (14), vanillic acid (15), ethyl heptadecanoate (16), 2-O-quebrachitol (17), 2, 3, 4-trihydroxy-isovaleric acid (18), stigmasterol (19), betulinic acid (20), heptadecanoic acid (21), β-sitosterol (22), and β-daucosterol (23). Conclusion: Compounds 10-19 are obtained from this plant for the first time.